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| Cormier, Bettie; Gambardella, Chiara; Tato, Tania; Perdriat, Quentin; Costa, Elisa; Veclin, Cloé; Le Bihanic, Florane; Grassl, Bruno; Dubocq, Florian; Kärrman, Anna; Van Arkel, Kim; Lemoine, Soazig; Lagarde, Fabienne; Morin, Benedicte; Garaventa, Francesca; Faimali, Marco; Cousin, Xavier; Bégout, Marie-laure; Beiras, Ricardo; Cachot, Jerome. |
| Microplastics are ubiquitous in aquatic ecosystems, but little information is currently available on the dangers and risks to living organisms. In order to assess the ecotoxicity of environmental microplastics (MPs), samples were collected from the beaches of two islands in the Guadeloupe archipelago, Petit-Bourg (PB) located on the main island of Guadeloupe and Marie-Galante (MG) on the second island of the archipelago. These samples have a similar polymer composition with mainly polyethylene (PE) and polypropylene (PP). However, these two samples are very dissimilar with regard to their contamination profile and their toxicity. MPs from MG contain more lead, cadmium and organochlorine compounds while those from PB have higher levels of copper, zinc and... |
| Tipo: Text |
Palavras-chave: Environmental microplastics; Leachates; Early life stages; Aquatic organisms; Toxicity. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00661/77276/78711.pdf |
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| Vignet, Caroline; Le Menach, Karyn; Mazurais, David; Lucas, Julie; Perrichon, Prescilla; Le Bihanic, Florane; Devier, Marie-hélène; Lyphout, Laura; Frere, Laura; Begout, Marie-laure; Zambonino-infante, Jose Luis; Budzinski, Helene; Cousin, Xavier. |
| The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel... |
| Tipo: Text |
Palavras-chave: Danio rerio; Life cycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Jaw; Digestion. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29907/28490.pdf |
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| Le Bihanic, Florane; Perrichon, Prescilla; Landi, Laure; Clerandeau, Christelle; Le Menach, Karyn; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome. |
| Most persistent organic pollutants, due to their hydrophobic properties, accumulate in aquatic sediments and represent a high risk for sediment quality. To assess the toxicity of hydrophobic pollutants, a novel approach was recently proposed as an alternative to replace, refine and reduce animal experimentation: the medaka embryo–larval sediment contact assay (MELAc). This assay is performed with Japanese medaka embryos incubated on a natural sediment spiked with the compound being tested. With the aim of improving this assay, our study developed a reference exposure protocol with an artificial sediment specifically designed to limit natural sediment composition uncertainties and preparation variability. The optimum composition of the new artificial... |
| Tipo: Text |
Palavras-chave: Artificial sediment; Spiked sediment; PAHs; Japanese medaka embryos; Embryotoxicity; Teratogenicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00187/29872/28479.pdf |
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| Le Bihanic, Florane; Morin, Benedicte; Cousin, Xavier; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome. |
| A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic... |
| Tipo: Text |
Palavras-chave: PAH mixture; Oil extract; Pyrolytic extract; Embryotoxicity; Developmental defects; Genotoxicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29919/28384.pdf |
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| Le Bihanic, Florane; Clerandeau, Christelle; Le Menach, Karyn; Morin, Benedicte; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome. |
| In aquatic environments, polycyclic aromatic hydrocarbons (PAHs) mostly occur as complex mixtures, for which risk assessment remains problematic. To better understand the effects of PAH mixture toxicity on fish early life stages, this study compared the developmental toxicity of three PAH complex mixtures. These mixtures were extracted from a PAH-contaminated sediment (Seine estuary, France) and two oils (Arabian Light and Erika). For each fraction, artificial sediment was spiked at three different environmental concentrations roughly equivalent to 0.5, 4, and 10 μg total PAH g−1 dw. Japanese medaka embryos were incubated on these PAH-spiked sediments throughout their development, right up until hatching. Several endpoints were recorded at different... |
| Tipo: Text |
Palavras-chave: Developmental abnormalities; Comet assay; Micronucleus assay; Larvae locomotion; Oil extract; Pyrolytic extract; Medaka embryo-larval assay. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29920/28385.pdf |
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| Le Bihanic, Florane; Sommard, Vivien; De Lansalut, Perrine; Pichon, Anaïk; Grasset, Julie; Berrada, Saadia; Budzinski, Helene; Cousin, Xavier; Morin, Benedicte; Cachot, Jerome. |
| Benz[a]anthracene (BaA) is a ubiquitous polycyclic aromatic hydrocarbon found in numerous aquatic ecosystems. However, ecotoxicological data in aquatic organisms are scarce. To remedy this lack of data, Japanese medaka (Oryzias latipes) embryos were exposed to BaA and toxic effects were investigated at multiple toxicological endpoints. Japanese medaka embryos were incubated onto BaA-spiked artificial sediment for 9 days at low or moderate environmental concentrations ranging from 0.9 to 12 µg g−1 dw. BaA-exposed embryos exhibited significant tachycardia. BaA exposure was also shown to increase CYP1A activity in the hepato-biliary tissue as well as craniofacial deformities and DNA damage in pro-larvae. The photomotor response of BaA-exposed larvae was... |
| Tipo: Text |
Palavras-chave: Benz[a]anthracene; Spiked sediment; Teratogenicity; DNA damage; Swimming behavior; In vivo EROD activity. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00244/35513/34729.pdf |
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| Perrichon, Prescilla; Le Bihanic, Florane; Bustamante, Paco; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome; Cousin, Xavier. |
| Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10-day zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals... |
| Tipo: Text |
Palavras-chave: Fish embryo-larval assay; PAHs; Artificial sediment; EROD; Behavioral performance; Teratogenicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00242/35323/37905.pdf |
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| Le Bihanic, Florane; Clérandeau, Christelle; Cormier, Bettie; Crebassa, Jean-claude; Keiter, Steffen H.; Beiras, Ricardo; Morin, Bénédicte; Bégout, Marie-laure; Cousin, Xavier; Cachot, Jerome. |
| The role of polyethylene microplastics 4–6 μm size (MPs) in the toxicity of environmental compounds to fish early life stages (ELS) was investigated. Marine medaka Oryzias melastigma embryos and larvae were exposed to suspended MPs spiked with three model contaminants: benzo(a)pyrene (MP-BaP), perfluorooctanesulfonic acid (MP-PFOS) and benzophenone-3 (MP-BP3) for 12 days. There was no evidence of MPs ingestion but MPs agglomerated on the surface of the chorion. Fish ELS exposed to virgin MPs did not show toxic effects. Exposure to MP-PFOS decreased embryonic survival and prevented hatching. Larvae exposed to MP-BaP or MP-BP3 exhibited reduced growth, increased developmental anomalies and abnormal behavior. Compared to equivalent waterborne concentrations,... |
| Tipo: Text |
Palavras-chave: Marine medaka; Microplastics; Developmental toxicity; Benzo(a)pyrene; Perfluorooctanesulfonic acid; Benzophenone-3. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00619/73112/74844.pdf |
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